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Germplasm Development at Panzea


  • Public grow outs - Although we are no longer conducting public grow outs of our maize NAM population, each summer that this project is active we will hold public grow outs of germplasm that is of interest both to us and to the maize community. Researchers are most welcome to visit these grow outs and score their own (non-destructive) traits of interest. For more information on these grow outs, contact us. In the summer of 2011 we will grow out, in five locations, two sets of hybrid germplasm:
    • Hybrids of 1400 NAM RILs crossed with the non-Stiff Stalk, ex-PVP tester PHZ51
    • Hybrids of 1200 maize inbred lines from the USDA Ames Plant Introduction Station collection (500 crossed with PHZ51 and 700 crossed with the Stiff Stalk tester PHB47)

  • Maize Nested Association Mapping (NAM) population - to form this population (consisting of 5000 RILs in total), 25 very diverse lines of maize were crossed to B73 and then 200 RILs were created from each of the 25 families, with each RIL derived from a unique F2 plant. A list of the 25 diverse maize inbred parental lines and their NCRPIS accession numbers can be obtained here. NAM is a new approach to the mapping of genes underlying complex traits, in which the statistical power of QTL mapping is combined with the high (potentially gene-level) chromosomal resolution of association mapping. The NAM concept is explained and demonstrated for the traits flowering time and leaf angle in the following publications:
    • Buckler ES, Holland JB, Bradbury PJ, Acharya CB, Brown PJ, Browne C, Ersoz E, Flint-Garcia S, Garcia A, Glaubitz JC, Goodman MM, Harjes C, Guill K, Kroon DE, Larsson S, Lepak NK, Li H, Mitchell SE, Pressoir G, Peiffer JA, Oropeza Rosas M, Rocheford TR, Romay MC, Romero S, Salvo S, Sanchez Villeda H, da Silva HS, Sun Q, Tian F, Upadyayula N, Ware D, Yates H, Yu J, Zhang Z, Kresovich S, McMullen MD. 2009. The genetic architecture of maize flowering time. Science 325: 714-718.   [pdf]   [download data]

    • Tian F, Bradbury PJ, Brown PJ, Hung H, Sun Q, Flint-Garcia S, Rocheford TR, McMullen MD, Holland JB, Buckler ES. 2011. Genome-wide association study of leaf architecture in the maize nested association mapping population. Nature Genetics 43: 159-162.   [pdf]   [download data]

    Also, for further explanation, see FAQ items What are RILs?, How are the NAM RILs useful? and What is Nested Association Mapping (NAM)?.

    SEED ARE NOW AVAILABLE for 4,835 of the maize NAM lines from the Maize Genetics Cooperation Stock Center. The remaining 165 lines will be available soon. The GENOTYPES AND GENETIC MAP for the NAM population for 1106 SNPs are available on our data sets page.

  • Maize Association Mapping Population - A total of 281 diverse inbred lines of maize from throughout the world, representing a wide range of genotypic and phenotypic diversity. A list of the lines and their NCRPIS accession numbers can be downloaded by clicking here. This population is described in detail in the following paper:
    • Flint-Garcia SA, Thuillet A-C, Romero SM, Yu J, Pressoir G, Mitchell S, Doebley J, Kresovich S, Goodman MM, Buckler ES. 2005. Maize association population: a high resolution platform for QTL dissection. Plant Journal, 44: 1054-1064.   [pdf]

    A Q-matrix describing the population structure of these lines (based on 95 SSR markers) is available here. Seed from these lines are currently available through NCRPIS - an up to date list of the NCRPIS accession numbers corresponding to these lines is available here. (See FAQ item What is association mapping?)
    Association panel genotypes at a set of 13 SSR markers (useful for quality control purposes) are available here.
  • 11 Teosinte Inbred Lines - We have developed a set of eleven teosinte inbred lines and have deposited seed with NCRPIS. The NCRPIS accession numbers for these are available here. Small quantities of seed from some of the lines can also be obtained from John Doebley (jdoebley_at_wisc.edu). (See FAQ item What is an inbred line?)
  • 23 Maize Landrace Inbreds - We have developed a set of 23 inbred lines from maize landraces originating from throughout the Americas. Seed have been deposited with NCRPIS. (See FAQ item What are maize landraces?)
  • Teosinte Association Mapping population - A collection of seed from 86 populations of teosinte (Zea mays ssp. parviglumis) for use in association mapping studies (e.g., Weber et al. 2007) has been deposited with NCRPIS. The NCRPIS accession numbers for these and additional passport information are available here.
  • 881 Maize-Teosinte Recombinant Inbred Lines (RILs) - BC2S3 population in a W22 background. For use in QTL mapping of domestication traits (See FAQ item What is QTL mapping?). The teosinte parent is Zea mays ssp. parviglumis accession CIMMYT 8759. Genotypes of these lines are available here. Seed are currently available from the Maize Genetics Cooperation Stock Center.


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